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Western blot analysis of GCDH using rabbit polyclonal GCDH Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCDH gene.
GCDH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of GCDH using rabbit polyclonal GCDH Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCDH gene.

GCDH Antibody

CATALOG NUMBER: 56-724

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey
Immunogen:
This GCDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-365 amino acids from the C-terminal region of human GCDH.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GCDH
Additional Names:
Glutaryl-CoA dehydrogenase, mitochondrial, GCD, GCDH
Protein Accession Number:
Q92947
Protein Gi Number:
2492631
Ncbi Gene Id Number:
2639
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the acyl-CoAdehydrogenase family. It catalyzes the oxidative decarboxylation ofglutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathwayof L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It useselectron transfer flavoprotein as its electron acceptor. The enzymeexists in the mitochondrial matrix as a homotetramer of 45-kDsubunits. Alternatively spliced transcript variants encodingdifferent isoforms have been identified.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Park, J.D., et al. J. Korean Med. Sci. 25(6):957-960(2010)
Background Reference 3:
Strauss, K.A., et al. Brain 133 (PT 1), 76-92 (2010) :
Background Reference 4:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-724

List Size:
400 ul

List Price:

$495.00

Datasheet
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