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45-661 staining (2ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-661 staining (2ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

GCIP Interacting Protein Antibody

CATALOG NUMBER: 45-661

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-AEIKQNLERGTAV
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26kDa band seen in Mouse Heart lysate and 30kDa band seen in Human Heart lysate (Predicted MW of 28kDa according to NP_056299). Recommended for use at 0.5-3ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SYF2
Additional Names:
GCIP-interacting protein p29, SYF2, SYF2 homolog, RNA splicing factor (S. cerevisiae), P29, CBPIN, NTC31, DKFZP564O2082, fSAP29, CCNDBP1 interactor, SYF2 homolog, RNA splicing factor, functional spliceosome-associated protein 29
Protein Accession Number:
NP_056299.1,NP_997053.1
Protein Gi Number:
7661636
Ncbi Gene Id Number:
25949
Background Reference 1:
Chang MS, Chang CL, Huang CJ, Yang YC. p29, a novel GCIP-interacting protein, localizes in the nucleus. Biochem Biophys Res Commun. 2000 Dec 20;279(2):732-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-661

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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