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Western blot analysis of hGCK-S418 in Jurkat cell line lysates (35ug/lane)
Western blot analysis of GCK using rabbit polyclonal hGCK-S418 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCK gene.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of hGCK-S418 in Jurkat cell line lysates (35ug/lane)

GCK Antibody

CATALOG NUMBER: 63-280

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 403-433 amino acids from the C-terminal region of human GCK.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GCK
Additional Names:
Glucokinase, Hexokinase type IV, HK IV, Hexokinase-4, HK4, Hexokinase-D, GCK
Protein Accession Number:
P35557
Protein Gi Number:
547696
Ncbi Gene Id Number:
2645
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2); mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Background Reference 1:
Gloyn, A.L., et al., Diabetes 52(9):2433-2440 (2003).
Background Reference 2:
Pruhova, S., et al., Diabetologia 46(2):291-295 (2003).
Background Reference 3:
Rizzo, M.A., et al., J. Biol. Chem. 277(37):34168-34175 (2002).
Background Reference 4:
Cao, H., et al., Hum. Mutat. 20(6):478-479 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-280

List Size:
400 ul

List Price:

$495.00

Datasheet
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