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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).

GCLC Antibody

CATALOG NUMBER: 59-915

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human GCLC.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
73 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GCLC
Additional Names:
Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCLC, GLCL, GLCLC
Protein Accession Number:
P48506
Protein Gi Number:
1346190
Ncbi Gene Id Number:
2729
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glutamate-cysteine ligase, also known asgamma-glutamylcysteine synthetase is the first rate-limiting enzymeof glutathione synthesis. The enzyme consists of two subunits, aheavy catalytic subunit and a light regulatory subunit. This locusencodes the catalytic subunit, while the regulatory subunit isderived from a different gene located on chromosome 1p22-p21.Mutations at this locus have been associated with hemolytic anemiadue to deficiency of gamma-glutamylcysteine synthetase andsusceptibility to myocardial infarction.
Background Reference 1:
Man, B.L., et al.Clin Neurosci 17(10):1244-1247(2010)
Background Reference 2:
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
Background Reference 3:
Le, T.M., et al. Mol. Genet. Metab. 101(1):55-61(2010)
Background Reference 4:
Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-915

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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