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Antibody used in WB on Mouse heart at: 1:1000 (Lane 1: 40ug mouse heart lysate, Lane 2: 40ug mouse heart lysate).
Antibody used in WB on Human MCF7 at 0.2-1 ug/ml.
Antibody used in IHC on Human Liver.
Antibody used in WB on Mouse heart at: 1:1000 (Lane 1: 40ug mouse heart lysate, Lane 2: 40ug mouse heart lysate).

GCLM Antibody

CATALOG NUMBER: 26-904

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GCLM.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
GCLM antibody can be used for detection of GCLM by ELISA at 1:312500. GCLM antibody can be used for detection of GCLM by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Predicted Molecular Weight:
31 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store GCLM antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
GCLM
Additional Names:
GCLM, GLCLR
Protein Accession Number:
NP_002052
Protein Gi Number:
4504011
Ncbi Gene Id Number:
2730
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background Reference 1:
Jonsson, L.S., (2008) Int Arch Occup Environ Health 81 (7), 913-919.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-904

List Size:
100 ul

List Price:

$519.00

Datasheet
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