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Western blot analysis of extracts of various cell lines, using GCN1L1 antibody (14-780) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using GCN1L1 antibody (14-780) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GCN1L1 antibody (14-780) at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using GCN1L1 antibody (14-780) at dilution of 1:150 (40x lens).
Western blot analysis of extracts of various cell lines, using GCN1L1 antibody (14-780) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

GCN1L1 Antibody

CATALOG NUMBER: 14-780

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2462-2671 of human GCN1L1 (NP_006827.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
OVCAR3
Positive Control 2:
U-87MG
Positive Control 3:
HepG2
Positive Control 4:
HeLa
Predicted Molecular Weight:
Observed: 265kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GCN1L1
Additional Names:
GCN1LL1, PRIC295, GCN1, eIF-2-alpha kinase activator GCN1, GCN1 (general control of amino-acid synthesis 1, yeast)-like 1, GCN1 eIF-2-alpha kinase activator homolog, GCN1 general control of amino-acid synthesis 1-like 1, GCN1, eIF2 alpha kinase activator homolog, GCN1-like protein 1, general control of amino-acid synthesis 1-like protein 1, hsGCN1, peroxisome proliferator activated receptor interacting complex protein, translational activator GCN1
Ncbi Gene Id Number:
10985
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-780

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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