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Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lane 3: mouse kidney lysate Lane 4: 293 whole cell lysate Lane 5: Hela whole cell lysate Lane 5: mouse brain lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with GDF11 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with GDF11 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: K562 whole cell lysate Lane 3: mouse kidney lysate Lane 4: 293 whole cell lysate Lane 5: Hela whole cell lysate Lane 5: mouse brain lysate Lysates/proteins at 20 ug per lane.

GDF11 Antibody

CATALOG NUMBER: 61-067

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This GDF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human GDF11.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GDF11
Additional Names:
Growth/differentiation factor 11, GDF-11, Bone morphogenetic protein 11, BMP-11, GDF11, BMP11
Protein Accession Number:
O95390
Ncbi Gene Id Number:
10220
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GDF11 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development.
Background Reference 1:
Lee, S.J., et al., Curr. Opin. Genet. Dev. 9(5):604-607 (1999).
Background Reference 2:
McPherron, A.C., et al., Nat. Genet. 22(3):260-264 (1999).
Background Reference 3:
Gamer, L.W., et al., Dev. Biol. 208(1):222-232 (1999).
Background Reference 4:
Hillier, L.D., et al., Genome Res. 6(9):807-828 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-067

List Size:
400 ul

List Price:

$495.00

Datasheet
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