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45-663 (0.3µg/ml) staining of Human Testes (A) and negative control Skin (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
HEK293 overexpressing GDF15 (RC201295) and probed with 45-663 (mock transfection in first lane)
45-663 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-663 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-663 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
45-663 (0.3µg/ml) staining of Human Testes (A) and negative control Skin (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

GDF15 Antibody

CATALOG NUMBER: 45-663

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-QKTDTGVSLQTYDD
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 30-35 kDa band observed in Human Testis lysates (calculated MW of 34.1kDa according to NP_004855.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Skin lysate. In transfected HEK293 transiently expressing GDF15 a band of approx. 35 kDa is observed. This band is not observed in the non-transfected HEK293. Preliminary testing was unsuccessful on Mouse for this particular batch. IHC: Paraffin embedded Human Prostate and Placenta. Recommended concentration: 5µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1312 - Human Prostate Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GDF15
Additional Names:
GDF15, PDF, MIC1, PLAB, MIC-1, NAG-1, PTGFB, GDF-15, growth differentiation factor 15, PTGF-beta, prostate differentiation factor, NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1
Protein Accession Number:
NP_004855.2
Protein Gi Number:
4758936
Ncbi Gene Id Number:
9518
Background Reference 1:
Wollmann W, Goodman ML, Bhat-Nakshatri P, Kishimoto H, Goulet RJ Jr, Meharotra S, Morimiya A, Badve S, Nakshatri H. The macrophage inhibitory cytokine integrates AKT/PKB and MAP kinase signaling pathways in breast cancer cells. Carcinogenesis. 2005 Jan 27;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-663

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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