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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
GDF15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

GDF15 Antibody

CATALOG NUMBER: 56-501

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GDF15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-79 amino acids from the N-terminal region of human GDF15.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GDF15
Additional Names:
Growth/differentiation factor 15, GDF-15, Macrophage inhibitory cytokine 1, MIC-1, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1, Placental TGF-beta, Placental bone morphogenetic protein, Prostate differentiation factor, GDF15, MIC1, PDF, PLAB, PTGFB
Protein Accession Number:
Q99988
Protein Gi Number:
313104195
Ncbi Gene Id Number:
9518
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bone morphogenetic proteins (e.g., BMP9; MIM 605120) aremembers of the transforming growth factor-beta (see TGFB1; MIM190180) superfamily and regulate tissue differentiation andmaintenance. They are synthesized as precursor molecules that areprocessed at a dibasic cleavage site to release C-terminal domainscontaining a characteristic motif of 7 conserved cysteines in themature protein.
Background Reference 1:
Anand, I.S., et al. Circulation 122(14):1387-1395(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Staff, A.C., et al. Gynecol. Oncol. 118(3):237-243(2010)
Background Reference 4:
Roth, P., et al. Clin. Cancer Res. 16(15):3851-3859(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-501

List Size:
400 ul

List Price:

$495.00

Datasheet
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