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Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: H. brain whole lysate Lane 4: M. brain whole lysate Lane 5: R. brain whole lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: U-87 MG whole cell lysate Lane 3: Hela whole cell lysate Lane 4: rat brain lysate Lane 5: mouse brain lysate Lane 6: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lane 3: H. brain whole lysate Lane 4: M. brain whole lysate Lane 5: R. brain whole lysate Lysates/proteins at 20 ug per lane.

GDI1 Antibody

CATALOG NUMBER: 60-860

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey
Immunogen:
This GDI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 415-443 amino acids from the C-terminal region of human GDI1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000-1:2000
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GDI1
Additional Names:
Rab GDP dissociation inhibitor alpha, Rab GDI alpha, Guanosine diphosphate dissociation inhibitor 1, GDI-1, Oligophrenin-2, Protein XAP-4, GDI1, GDIL, OPHN2, RABGDIA, XAP4
Protein Accession Number:
P31150
Protein Gi Number:
1707886
Ncbi Gene Id Number:
2664
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GDP dissociation inhibitors are proteins that regulate theGDP-GTP exchange reaction of members of the rab family, smallGTP-binding proteins of the ras superfamily, that are involved invesicular trafficking of molecules between cellular organelles.GDIs slow the rate of dissociation of GDP from rab proteins andrelease GDP from membrane-bound rabs. GDI1 is expressed primarilyin neural and sensory tissues. Mutations in GDI1 have been linkedto X-linked nonspecific mental retardation.
Background Reference 1:
Martins-de-Souza, D., et al.Psychiatr Res 44(14):989-991(2010)
Background Reference 2:
Massignan, T., et al. Mol. Cell Proteomics 9(4):611-622(2010)
Background Reference 3:
Chen, Y., et al. Biochem. J. 422(2):229-235(2009)
Background Reference 4:
Zhang, K.J., et al. Yi Chuan 30(5):590-594(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-860

List Size:
400 ul

List Price:

$495.00

Datasheet
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