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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
GDI2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

GDI2 Antibody

CATALOG NUMBER: 57-273

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GDI2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human GDI2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GDI2
Additional Names:
Rab GDP dissociation inhibitor beta, Rab GDI beta, Guanosine diphosphate dissociation inhibitor 2, GDI-2, GDI2, RABGDIB
Protein Accession Number:
P50395
Protein Gi Number:
13638228
Ncbi Gene Id Number:
2665
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GDP dissociation inhibitors are proteins that regulate theGDP-GTP exchange reaction of members of the rab family, smallGTP-binding proteins of the ras superfamily, that are involved invesicular trafficking of molecules between cellular organelles.GDIs slow the rate of dissociation of GDP from rab proteins andrelease GDP from membrane-bound rabs. GDI2 is ubiquitouslyexpressed. The GDI2 gene contains many repetitive elementsindicating that it may be prone to inversion/deletionrearrangements. Alternative splicing results in multiple transcriptvariants encoding distinct isoforms.
Background Reference 1:
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Background Reference 2:
Sun, Z.L., et al. Biochim. Biophys. Acta 1774(6):764-771(2007)
Background Reference 3:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 4:
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-273

List Size:
400 ul

List Price:

$495.00

Datasheet
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