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HEK293 lysate overexpressing full-length Human GEM (HA tagged), mock-transfected HEK293 (EV) and HEK293 transiently expressing GEM-related genes (Rem, Rem2L and Rad) probed with 43-052 (1ug/ml). The same lysates probed with anti-HA tag antibody in the lo
43-052 (1ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 lysate overexpressing full-length Human GEM (HA tagged), mock-transfected HEK293 (EV) and HEK293 transiently expressing GEM-related genes (Rem, Rem2L and Rad) probed with 43-052 (1ug/ml). The same lysates probed with anti-HA tag antibody in the lo

GEM Antibody

CATALOG NUMBER: 43-052

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QKEPHQYSHRNRH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing Human recombinant GEM bands of approx. 35+45kDa are observed. These bands are not observed in the transfected HEK293 with empty vector and the antibody does not cross-react to related protein such as Rem, Rem2L or Rad. Data provided by Prof. R Andres, Univ. Kentucky, USA. A similar banding pattern we observed in Human Spleen. The calculated molecular size is 33.9kDa according to NP_005252.1. Recommended concentration: 1-3ug/ml.
Specificity:
Reported variants represent identical protein: NP_005252.1, NP_859053.1
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GEM
Additional Names:
GEM, GTP binding protein overexpressed in skeletal muscle, KIR, MGC26294, GTP-binding mitogen-induced T-cell protein, GTP-binding protein GEM, GTP-binding protein overexpressed in skeletal muscle, OTTHUMP00000226600, OTTHUMP00000226601, RAS-like protein KIR, kinase-inducible Ras-like protein
Protein Accession Number:
NP_005252.1
Protein Gi Number:
4885263
Ncbi Gene Id Number:
2669

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-052

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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