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Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of lysate from MDA-MB-453 cell line, using GEN1 Antibody at 1:1000.
Western blot analysis of GEN1 Antibody in MDA-MB435 cell line lysates (35ug/lane).
Western blot analysis of GEN1 Antibody in mouse liver tissue lysates (35ug/lane)
Western Blot at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

GEN1 Antibody

CATALOG NUMBER: 63-976

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This GEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human GEN1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
103 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GEN1
Additional Names:
Flap endonuclease GEN homolog 1, 31--, GEN1
Protein Accession Number:
Q17RS7
Protein Gi Number:
290457644
Ncbi Gene Id Number:
348654
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-stranded DNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded. Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strand overhang of the flap is double-stranded (By similarity).
Background Reference 1:
Ip, S.C., et al. Nature 456(7220):357-361(2008)
Background Reference 2:
Ishikawa, G., et al. Nucleic Acids Res. 32(21):6251-6259(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-976

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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