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Antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Antibody is used in Western blot to detect MAP4K2 in mouse skeletal muscle tissue lysate.

Germinal Center Kinase Antibody

CATALOG NUMBER: 62-630

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Germinal Center Kinase (GCK/MAP4K2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-319 amino acids from the Central region of human Germinal Center Kinase (GCK/MAP4K2).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
92 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP4K2
Additional Names:
Mitogen-activated protein kinase kinase kinase kinase 2, B lymphocyte serine/threonine-protein kinase, Germinal center kinase, GC kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, MEKKK 2, Rab8-interacting protein, MAP4K2, GCK, RAB8IP
Protein Accession Number:
Q12851
Protein Gi Number:
215274019
Ncbi Gene Id Number:
5871
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.
Background Reference 1:
Chadee, D.N., et al., Mol. Cell. Biol. 22(3):737-749 (2002).
Background Reference 2:
Katz, P., et al., J. Biol. Chem. 269(24):16802-16809 (1994).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-630

List Size:
400 ul

List Price:

$495.00

Datasheet
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