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45-063 (5ug/ml) as the reporter antibody.
45-063 (2ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-063 (0.01ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Ex
45-063 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-063 (0.1ug/ml) staining of Mouse (A) and (0.01ug/ml) Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-063 (5ug/ml) as the reporter antibody.

GFAP Antibody

CATALOG NUMBER: 45-063

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog
Immunogen:
The immunogen for this antibody is: C-DGEVIKESKQEHKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa observed in Mouse and Rat Brain lysates (calculated MW of 49.9kDa according to Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration:0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.
Specificity:
GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GFAP
Additional Names:
GFAP, glial fibrillary acidic protein , FLJ45472 , intermediate filament protein
Protein Accession Number:
NP_002046.1
Protein Gi Number:
4503979
Ncbi Gene Id Number:
2670
Background Reference 1:
Korolainen MA, Auriola S, Nyman TA, Alafuzoff I, Pirttila T. Proteomic analysis of glial fibrillary acidic protein in Alzheimer's disease and aging brain. Neurobiol Dis. 2005 Dec;20(3):858-70. Epub 2005 Jun 27.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-063

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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