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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).

GFER Antibody

CATALOG NUMBER: 55-976

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This GFER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-202 amino acids from the C-terminal region of human GFER.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GFER
Additional Names:
FAD-linked sulfhydryl oxidase ALR, Augmenter of liver regeneration, hERV1, Hepatopoietin, GFER, ALR, HERV1, HPO
Protein Accession Number:
P55789
Protein Gi Number:
218511915
Ncbi Gene Id Number:
2671
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The hepatotrophic factor designated augmenter of liverregeneration (ALR) is thought to be one of the factors responsiblefor the extraordinary regenerative capacity of mammalian liver. Ithas also been called hepatic regenerative stimulation substance(HSS). The gene resides on chromosome 16 in the interval containingthe locus for polycystic kidney disease (PKD1). The putative geneproduct is 42% similar to the scERV1 protein of yeast. The yeastscERV1 gene had been found to be essential for oxidativephosphorylation, the maintenance of mitochondrial genomes, and thecell division cycle. The human gene is both the structural andfunctional homolog of the yeast scERV1 gene.
Background Reference 1:
Dong, L.Y., et al. Biochem. J. 431(2):277-287(2010)
Background Reference 2:
Li, W., et al. FEBS Lett. 584(18):3929-3935(2010)
Background Reference 3:
Daithankar, V.N., et al. Biochemistry 49(31):6737-6745(2010)
Background Reference 4:
Dayoub, R., et al. Biochem. Biophys. Res. Commun. 395(4):465-470(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-976

List Size:
400 ul

List Price:

$495.00

Datasheet
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