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GFP Antibody

CATALOG NUMBER: XR-9001

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
GFP
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
GFP
Immunogen:
Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Recommended dilutions are as follows, ELISA 1:15,000 - 1:60,000, Western Blot 1:500 - 1:5,000, Immunohistochemistry 1:200 - 1:3,000. (Optimal dilutions should be determined by user).
Purification:
Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2
Concentration:
1.0 mg/mL
Storage Conditions:
Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. Forextended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
Ncbi Official Symbol:
gfp
Additional Names:
Green fluorescent protein
Protein Accession Number:
YP_002302326.1
Protein Gi Number:
211909965
Ncbi Gene Id Number:
7011691
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

XR-9001

List Size:
0.1 mg

List Price:

$442.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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