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Western blot analysis of GFPT2 Antibody in A2058, K562 cell line lysates (35ug/lane)
GFPT2 Antibody IHC analysis in formalin fixed and paraffin embedded human skin followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of GFPT2 Antibody in A2058, K562 cell line lysates (35ug/lane)

GFPT2 Antibody

CATALOG NUMBER: 63-873

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This GFPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-201 amino acids from the Central region of human GFPT2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
77 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GFPT2
Additional Names:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2, D-fructose-6-phosphate amidotransferase 2, Glutamine:fructose-6-phosphate amidotransferase 2, GFAT 2, GFAT2, Hexosephosphate aminotransferase 2, GFPT2
Protein Accession Number:
O94808
Ncbi Gene Id Number:
9945
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GFPT2 controls the flux of glucose into the hexosamine pathway. This protein most likely involved in regulating the availability of precursors for N-and O-linked glycosylation of proteins.
Background Reference 1:
Edwards,T.L., et.al., Ann. Hum. Genet. 74 (2), 97-109 (2010)
Background Reference 2:
Prasad,P., et.al., BMC Med. Genet. 11, 52 (2010)
Background Reference 3:
Srinivasan,V., et.al., Clin. Biochem. 40 (13-14), 952-957 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-873

List Size:
400 ul

List Price:

$495.00

Datasheet
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