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Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1 μg/mL.
Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 2.5 μg/ml.
Immunofluorescence of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 20 μg/mL.<br><br>Red: GFR alpha 1 Antibody (1133) <br> Blue: DAPI staining
Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 μg/mL.
Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1 μg/mL.
Trial Size

GFR alpha 1 Antibody

CATALOG NUMBER: 1133

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GFR alpha 1 antibody was raised against a 14 amino acid peptide near the center of human GFR alpha 1.
The immunogen is located within amino acids 360 - 410 of GFR alpha 1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GFR alpha 1 antibody can be used for detection of GFR alpha 1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GFR alpha 1 antibody is predicted to not cross-react with other members of the GFR alpha family of proteins.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 3:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 4:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 5:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
51 kDa
Purification:
GFR alpha 1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GFR alpha 1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GFR alpha 1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GFRA1
Additional Names:
GFR alpha 1 Antibody: GDNFR, RET1L, RETL1, TRNR1, GDNFRA, GFR-ALPHA-1, GDNF family receptor alpha-1, RET ligand 1, GDNF receptor alpha-1
Protein Accession Number:
P56159
Protein Gi Number:
20141405
Ncbi Gene Id Number:
2674
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GFR alpha 1 Antibody: Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRα-1 recently. GFRα-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRα and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.
Background Reference 1:
Jing S, Wen D, Yu Y, et al. GDNF-induced activation of the Ret protein tyrosine kinase is mediated by GDNFR-a, a novel receptor for GDNF. Cell 1996; 85:1113-24.
Background Reference 2:
Treanor JJS, Goodman L, Sauvage FD, et al. Characterization of a multicomponent receptor for GDNF. Nature 1996;82:80-83.
Background Reference 3:
Sanicola M, Hession C, Worley D, et al. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc. Natl. Acad. Sci. USA 1997; 94:6238-43.
Background Reference 4:
Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997; 387:721-4

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

1133

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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