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Western blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1 μg/mL.
Immunocytochemistry of GFR alpha 2 in HeLa cells with GFR alpha 2 antibody at 5 μg/mL.
Western blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1 μg/mL.
Trial Size

GFR alpha 2 Antibody

CATALOG NUMBER: 1135

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
GFR alpha 2 antibody was raised against a 15 amino acid peptide near the center of human GFR alpha 2.
The immunogen is located within amino acids 360 - 410 of GFR alpha 2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
GFR alpha 2 antibody can be used for detection of GFR alpha 2 by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 51 kDa
Observed: 52 kDa
Purification:
GFR alpha 2 Antibody is Ion exchange chromatography purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GFR alpha 2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
GFR alpha 2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GFRA2
Additional Names:
GFR alpha 2 Antibody: NTNRA, RETL2, TRNR2, GDNFRB, NRTNR-ALPHA, GDNF family receptor alpha-2, GDNF receptor beta, GDNF receptor alpha-2
Protein Accession Number:
AAC52036
Protein Gi Number:
2145080
Ncbi Gene Id Number:
2675
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GFR alpha 2 Antibody: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRalpha, which is a cell surface receptor for NTN. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently. NTNRalpha was also termed GDNFRβ, Ret ligand 2 (RETL2) or TGFbeta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. GFRα-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRα-2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function.
Background Reference 1:
Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996; 384:467-70.
Background Reference 2:
Klein RD, Sherman D, Ho WH, et al. A GPI-linked protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997; 387:717-21.
Background Reference 3:
Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997; 387:721-4.
Background Reference 4:
Baloh RH, Tansey MG, Golden JP, et al. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997; 18:793-802.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

1135

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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