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Western blot analysis of GFR alpha 3 in crude cell membrane fractions of mouse heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with GFR alpha 3 antibody at 1 2 μg/mL.
Immunohistochemistry of GFR alpha 3 in mouse heart tissue with GFR alpha 3 antibody at 10 μg/mL.
Immunofluorescence of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 μg/ml. <br><br>Green: GFR alpha 3 antibody (1137) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Immunohistochemistry of GFR alpha 3 in mouse kidney tissue with GFR alpha 3 antibody at 5 μg/ml.
Western blot analysis of GFR alpha 3 in crude cell membrane fractions of mouse heart (H), spleen (S), kidney (K), liver (L), and brain (B), respectively, with GFR alpha 3 antibody at 1 2 μg/mL.
Trial Size

GFR alpha 3 Antibody

CATALOG NUMBER: 1137

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GFR alpha 3 antibody was raised against a 14 amino acid peptide near the center of murine GFR alpha 3.
The immunogen is located within the last 50 amino acids of GFR alpha 3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GFR alpha 3 antibody can be used for detection of GFR alpha 3 expression by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 2:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Predicted Molecular Weight:
Predicted: 44 kDa
Observed: 43 kDa
Purification:
GFR alpha 3 Antibody is DEAE purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GFR alpha 3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
GFR alpha 3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Gfra3
Additional Names:
GFR alpha 3 Antibody: Y15110, GFRalpha3, GDNF family receptor alpha-3, GDNF receptor alpha-3
Protein Accession Number:
NP_034410
Protein Gi Number:
160298126
Ncbi Gene Id Number:
14587
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GFR alpha 3 Antibody: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play essential roles in the control of vertebrate neuron survival and differentiation. A new member of the GDNF family was recently identified and designated persephin. Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRα and Ret receptor tyrosine kinase GFRbeta. Following the findings of GFRα-1 and -2, a novel receptor in GFRα family was identified from human and mouse and designated GFRα-3. GFRα-3 binds persephin, thus, persephin, GFRα-3, and Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
Background Reference 1:
Milbrandt J, de Sauvage FJ, Fahrner TJ, et al. Persephin, a novel neurotrophic factor related to GDNF and neurturin. Neuron 1998; 20:245-253.
Background Reference 2:
Nomeclature Committee. Nomeclature of GPI-linked receptors for the GDNF ligand family. GFRa. Neuron 1997; 19:485.
Background Reference 3:
Jing S, Yu Y, Fang M, et al. GFRα-2 and GFRα-3 are two new receptors for ligands of the DGNF family. J. Biol. Chem. 1997; 272:33111-7.
Background Reference 4:
Baloh RH, Gorodinsky A, Golden JP, et al. GFRα3 is an orphan member of the GDNF/neurturin/persephin receptor family. Proc. Natl. Acad. Sci. USA 1998; 95:5801-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

1137

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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