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Western blot analysis of extracts of various cell lines, using GGA2 antibody (14-174) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human kidney using GGA2 Antibody (14-174) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using GGA2 Antibody (14-174) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GGA2 Antibody (14-174) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using GGA2 antibody (14-174). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using GGA2 antibody (14-174) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

GGA2 Antibody

CATALOG NUMBER: 14-174

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GGA2 (NP_055859.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HepG2
Positive Control 2:
Raji
Positive Control 3:
MCF7
Positive Control 4:
THP-1
Positive Control 5:
22Rv1
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 72-80kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GGA2
Additional Names:
GGA2, KIAA1080, VEAR
Ncbi Gene Id Number:
23062
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-174

List Size:
100 uL

List Price:

$436.00

Datasheet
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