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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
GGH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of GGH Antibody with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

GGH Antibody

CATALOG NUMBER: 56-199

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GGH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the C-terminal region of human GGH.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GGH
Additional Names:
Gamma-glutamyl hydrolase, Conjugase, GH, Gamma-Glu-X carboxypeptidase, GGH
Protein Accession Number:
Q92820
Protein Gi Number:
6016127
Ncbi Gene Id Number:
8836
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene catalyzes the hydrolysis offolylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates bythe removal of gamma-linked polyglutamates and glutamate. [providedby RefSeq].
Background Reference 1:
Adjei, A.A., et al.Thorac Oncol 5(9):1346-1353(2010)
Background Reference 2:
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Background Reference 3:
Organista-Nava, J., et al. Leuk. Res. 34(6):728-732(2010)
Background Reference 4:
Figueiredo, J.C., et al. Cancer Causes Control 21(4):597-608(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-199

List Size:
400 ul

List Price:

$495.00

Datasheet
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