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Western blot analysis of extracts of various cell lines, using GGT1 antibody (18-088) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using GGT1 antibody (18-088) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using GGT1 antibody (18-088) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using GGT1 antibody (18-088) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using GGT1 antibody (18-088) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

GGT1 Antibody

CATALOG NUMBER: 18-088

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 370-569 of human GGT1 (NP_001027537.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
Mouse kidney
Predicted Molecular Weight:
Observed: 60-70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GGT1
Additional Names:
GGT1, gamma-glutamyltransferase 1, CD224, D22S672, D22S732, GGT, GGT 1, GTG, gamma-glutamyl transpeptidase, gamma-glutamyltranspeptidase 1, glutamyl transpeptidase, glutathione hydrolase 1
Ncbi Gene Id Number:
2678
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-088

List Size:
100 uL

List Price:

$436.00

Datasheet
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