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Western blot analysis in U251 cell line lysates (35ug/lane).
GGTLC2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in U251 cell line lysates (35ug/lane).

GGTLC2 Antibody

CATALOG NUMBER: 55-092

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat
Immunogen:
This GGTLC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-154 amino acids from the Central region of human GGTLC2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GGTLC2
Additional Names:
Gamma-glutamyltransferase light chain 2, Gamma-glutamyltransferase-like protein 4, GGTLC2, GGTL4
Protein Accession Number:
Q14390
Protein Gi Number:
294862536
Ncbi Gene Id Number:
91227
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gamma-glutamyltransferase-1 (GGT1; MIM 612346) is amembrane-bound extracellular enzyme that cleaves gamma-glutamylpeptide bonds in glutathione and other peptides and transfers thegamma-glutamyl moiety to acceptors. Autocatalytic cleavage of theGGT1 precursor polypeptide produces a heavy chain and a light chainthat associate with each other to form the functional enzyme. Lightchain-only GGTs, such as GGTLC2, contain a region corresponding tothe GGT1 light chain, but they lack the membrane-anchoring heavychain region (Heisterkamp et al., 2008 [PubMed 18357469]).[suppliedby OMIM].
Background Reference 1:
Heisterkamp, N., et al. Hum. Genet. 123(4):321-332(2008)
Background Reference 2:
Dunham, I., et al. Nature 402(6761):489-495(1999)
Background Reference 3:
Kawasaki, K., et al. Genome Res. 7(3):250-261(1997)
Background Reference 4:
Leh, H., et al. FEBS Lett. 394(3):258-262(1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-092

List Size:
400 ul

List Price:

$495.00

Datasheet
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