Western blot analysis in K562 cell line lysates (35ug/lane).

GIF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of GIF Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).

GIF Antibody

CATALOG NUMBER: 56-771

Clonality:

Polyclonal

Tested Applications:

Flow, IF, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Immunogen:

This GIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the Central region of human GIF.

Conjugate:

Unconjugated

Tested Applications:

Flow, IF, IHC-P, WB

Application Note:

For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50

Predicted Molecular Weight:

45 kDa

Advanced Validation

Properties

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

GIF

Additional Names:

Gastric intrinsic factor, Intrinsic factor, IF, INF, GIF, IFMH

Protein Accession Number:

P27352

Protein Gi Number:

62906845

Ncbi Gene Id Number:

2694

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene is a member of the cobalamin transport proteinfamily. It encodes a glycoprotein secreted by parietal cells of thegastric mucosa and is required for adequate absorption of vitaminB12. Vitamin B12 is necessary for erythrocyte maturation andmutations in this gene may lead to congenital pernicious anemia.

Background Reference 1:

Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010)

Background Reference 2:

Andersen, C.B., et al. Nature 464(7287):445-448(2010)

Background Reference 3:

Ament, A.E., et al. Br. J. Haematol. 144(4):622-624(2009)

Background Reference 4:

Remacha, A.F., et al. Ann. Hematol. 87(7):599-600(2008)

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-771

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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GIF Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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