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45-064 staining (0.03ug/ml) of Human HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
HEK293 overexpressing GIPC1 isoform 1 and probed with 45-064 (mock transfection in first lane).
45-064 staining (0.03ug/ml) of Human HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

GIPC1 Antibody

CATALOG NUMBER: 45-064

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-GAIGDAKVGRY
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, and approx 24kDa also observed in Human Pancreas, Kidney and Muscle lysates (calculated MW of 36.0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.
Specificity:
This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1).
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GIPC1
Additional Names:
GIPC1, NIP, GIPC PDZ domain containing family, member 1, C19orf3, GIPC, GLUT1CBP, Hs.6454, IIP-1, MGC15889, MGC3774, RGS19IP1, SEMCAP, SYNECTIIN, TIP-2, GLUT1 C-terminal binding protein, IGF-1 receptor interacting protein 1, regulator of G-protein signalling 19 interacting protein 1, synectin, SYNECTIN
Protein Accession Number:
NP_005707.1,NP_974197.1,NP_974199.1,NP_974196.1,NP_974198.1,NP_974223.1
Protein Gi Number:
5031715
Ncbi Gene Id Number:
10755
Background Reference 1:
De Vries L, Lou X, Zhao G, Zheng B, Farquhar MG. GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP. Proc Natl Acad Sci USA. 1998 Oct 13;95(21):12340-5.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-064

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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