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Western blot analysis of Girdin in rat brain tissue lysate with Girdin antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of Girdin in human brain tissue with Girdin antibody at 20 μg/mL.
Western blot analysis of Girdin in rat brain tissue lysate with Girdin antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Girdin Antibody

CATALOG NUMBER: 5545

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Girdin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus human Girdin.
The immunogen is located within the last 50 amino acids of Girdin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Girdin antibody can be used for detection of Girdin by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
Girdin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Girdin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Girdin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CCDC88A
Additional Names:
Girdin Antibody: APE, GIV, GRDN, HkRP1, GIRDIN, KIAA1212, APE, Girdin, Akt phosphorylation enhancer
Protein Accession Number:
NP_060554
Protein Gi Number:
50897852
Ncbi Gene Id Number:
55704
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Girdin Antibody: Girdin is a Galpha-interacting protein that can enhance the activation of the protein kinase Akt, remodel the actin cytoskeleton, and is thought to be involved in the regulation of cell migration and cancer metastasis. It has recently been shown that Girdin interacts with Disrupted-in-Schizophrenia 1 (DISC1), a susceptibility gene for major psychiatric disorders. DISC1 is thought to be involved in the migration, positioning and differentiation of dentate granule cells (DGCs) during development; depletion of Girdin or blocking the Girdin-DISC1 interaction results in defects in axonal sprouting in the CA3 region of the hippocampus and overextended migration of mispositioning of DGCs, suggesting the Girdin plays a role in postnatal neurogenesis in the dentate gyrus.
Background Reference 1:
Le-Niculescu H, Niesman I, Fischer T, et al. Identification and characterization of GIV, a novel Galpha i/s-interacting protein found on COPI, endoplasmic reticulum-Golgi transport vesicles. J. Biol. Chem.2005; 280:22012-20.
Background Reference 2:
Anai M, Shojima N, Katagiri H, et al. A novel protein kinase B (PKB)/Akt-binding protein enhances PKB kinase activity and regulates DNA synthesis. J. Biol. Chem.2005; 280:18525-35.
Background Reference 3:
Enomoto A, Murakami H, Asai N, et al. Akt/PKB regulated actin organization and cell motility via Girdin/APE. Dev. Cell2005; 9:389-402.
Background Reference 4:
Enomoto A, Asai N, Namba T, et al. Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus. Neuron2009; 63:774-87.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5545

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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