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Western Blot at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.
GIT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: HUVEC whole cell lysate Lysates/proteins at 20 ug per lane.

GIT1 Antibody

CATALOG NUMBER: 62-896

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This GIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 485-512 amino acids from the Central region of human GIT1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
84 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GIT1
Additional Names:
ARF GTPase-activating protein GIT1, ARF GAP GIT1, Cool-associated and tyrosine-phosphorylated protein 1, CAT-1, CAT1, G protein-coupled receptor kinase-interactor 1, GRK-interacting protein 1, GIT1
Protein Accession Number:
Q9Y2X7
Protein Gi Number:
45645212
Ncbi Gene Id Number:
28964
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. It increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia.
Background Reference 1:
Sato,H., Biochem. Biophys. Res. Commun. 368 (1), 157-161 (2008)
Background Reference 2:
Jones,N.P., Mol. Cell. Biol. 27 (16), 5790-5805 (2007)
Background Reference 3:
Schmalzigaug,R., Cell. Signal. 19 (8), 1733-1744 (2007)
Background Reference 4:
Stockton,R., Mol. Biol. Cell 18 (6), 2346-2355 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-896

List Size:
400 ul

List Price:

$495.00

Datasheet
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