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<strong>Figure 1 WB Validation with Recombinant Protein </strong><br>Loading: GITRL recombinant protein lysate Antibodies: GITRL, 3591, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: 5 ng GITRL ProteinLane B: 25 ng GITRL ProteinLane C: 50 ng GITRL Protein
<strong>Figure 2 WB Validation in Human Pancreas </strong><br>Loading: 10 μg of lysate Antibodies: GITRL, 3591, 4 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 WB Validation in Mouse Spleen </strong><br>Loading: 10 μg of lysate Antibodies: GITRL, 3591, 4 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 WB Validation with Recombinant Protein
Loading: GITRL recombinant protein lysate Antibodies: GITRL, 3591, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: 5 ng GITRL ProteinLane B: 25 ng GITRL ProteinLane C: 50 ng GITRL Protein
Trial Size

GITRL Antibody

CATALOG NUMBER: 3591

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
GITRL antibody was raised against purified recombinant human GITR ligand.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 1-4 μg/mL

Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Positive Control 2:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Predicted Molecular Weight:
Predicted: 23 kD

Observed: 27 kD
Purification:
GITRL Antibody is Protein A purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GITRL Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GITRL antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TNFSF18
Additional Names:
GITRL Antibody: TL6, AITRL, GITRL, hGITRL, TL6, UNQ149/PRO175, Tumor necrosis factor ligand superfamily member 18, Activation-inducible TNF-related ligand
Protein Accession Number:
Q9UNG2
Protein Gi Number:
13124621
Ncbi Gene Id Number:
8995
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GITRL Antibody: The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions. Its ligand GITRL is expressed in endothelial and antigen-presenting cells and can activate NF-κB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR. Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-κB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.
Background Reference 1:
Gaur U, Aggarwal BB. Regulation of proliferation, survival and apoptosis by members of the TNF superfamily. Biochem. Pharmacol. 2003; 66:1403-8.
Background Reference 2:
Ronchetti S, Nocentini G, Riccardi C, et al. Role of GITR in activation response of T lymphocytes.Blood 2002; 100:350-2.
Background Reference 3:
Shimizu J, Yamakai S, Takahashi T, et al. Stimulation of CD25(+) CD4(+) regulatory T cells through GITR breaks immunological self- tolerance. Nat. Immunol. 2002;3:135-42.
Background Reference 4:
Gurney AL, Marsters SA, Huang A, et al. Identification of a new member of the tumor necrosis factor family and its receptor, a human ortholog of mouse GITR. Curr. Biol. 1999; 9:215-218.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3591

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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