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46-995 (0.1ug/ml) staining of Human (A) and (1ug/ml) Rat (B) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-995 (0.1ug/ml) staining of Human (A) and (1ug/ml) Rat (B) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GJA1 Antibody

CATALOG NUMBER: 46-995

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-QPFDFPDDNQNSKK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in Human and Rat Heart lysates (calculated MW of 43.0kDa according to Human NP_000156.1and Rat NP_036699.1). Recommended concentration: 0.1-1ug/ml. Primary incubation was 1 hour.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GJA1
Additional Names:
GJA1, gap junction protein, alpha 1, 43kDa, CX43, DFNB38, GJAL, ODDD, connexin 43, gap junction 43 kDa heart protein, gap junction protein, alpha-like
Protein Accession Number:
NP_000156.1
Protein Gi Number:
4504001
Ncbi Gene Id Number:
2697
Background Reference 1:
de la Parra DR, Zenteno JC. A new GJA1 (connexin 43) mutation causing oculodentodigital dysplasia associated to uncommon features. Ophthalmic Genet. 2007 Dec;28(4):198-202.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-995

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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