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Western blot analysis of GJA10 Antibody in mouse heart tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of GJA10 Antibody in mouse heart tissue lysates (35ug/lane)

GJA10 Antibody

CATALOG NUMBER: 58-186

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This GJA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-515 amino acids from the C-terminal region of human GJA10.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GJA9
Additional Names:
Gap junction alpha-9 protein, Connexin-58, Cx58, Connexin-59, Cx59, Gap junction alpha-10 protein, GJA9, GJA10
Protein Accession Number:
P57773
Protein Gi Number:
50403749
Ncbi Gene Id Number:
81025
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GJA8 is a an integral membrane protein that belongs to the connexin family, alpha-type (group II) subfamily. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.
Background Reference 1:
Hombach S, et al., EurNeurosci. 2004 May;19(10):2633-40.
Background Reference 2:
Houghton FD, et al., Biol Reprod. 2002 May;66(5):1403-12.
Background Reference 3:
Manthey D, et al.,Biol Chem. 1999 May 21;274(21):14716-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-186

List Size:
400 ul

List Price:

$495.00

Datasheet
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