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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJB5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GJB5 antibody detected the GJB5 protein (arrow).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJB5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

GJB5 Antibody

CATALOG NUMBER: 58-180

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This GJB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the Central region of human GJB5.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GJB5
Additional Names:
Gap junction beta-5 protein, Connexin-311, Cx311, GJB5
Protein Accession Number:
O95377
Ncbi Gene Id Number:
2709
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) such as GJB5 are thought to play crucial functional roles associated with these channels.
Background Reference 1:
Richard, G., et al., Nat. Genet. 20(4):366-369 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-180

List Size:
400 ul

List Price:

$495.00

Datasheet
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