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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
GKAP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of GKAP1 using rabbit polyclonal GKAP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GKAP1 gene.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

GKAP1 Antibody

CATALOG NUMBER: 61-941

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This GKAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-359 amino acids from the C-terminal region of human GKAP1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GKAP1
Additional Names:
G kinase-anchoring protein 1, cGMP-dependent protein kinase-anchoring protein of 42 kDa, GKAP1, GKAP42
Protein Accession Number:
Q5VSY0
Protein Gi Number:
166224631
Ncbi Gene Id Number:
80318
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GKAP1 is a protein that is highly similar to themouse cGMP-dependent protein kinase anchoring protein 42kDa. Themouse protein has been found to localize with the Golgi and recruitcGMP-dependent protein kinase I alpha to the Golgi in mouse testes.It is thought to play a role in germ cell development. Transcriptvariants encoding different isoforms have been found for this gene.
Background Reference 1:
Lim, J., et al. Cell 125(4):801-814(2006)
Background Reference 2:
Sweetser, D.A., et al. Genes Chromosomes Cancer 44(3):279-291(2005)
Background Reference 3:
Yuasa, K., et al. J. Biol. Chem. 275(7):4897-4905(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-941

List Size:
400 ul

List Price:

$495.00

Datasheet
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