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45-674 Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
45-674 Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

GLI2 Antibody

CATALOG NUMBER: 45-674

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: DLKEDLDRDDCKQE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Preliminary experiments gave an approx 55kDa band in Human Bone Marrow and Ovary lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 168kDa according to NP_005261.2. The 55kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Lymph Node shows nuclear staining in macrophages. Recommended concentration: 4-6ug/ml.
Specificity:
This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GLI2
Additional Names:
GLI2, GLI-Kruppel family member GLI2 , THP2, oncogene GLI2, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, zinc finger protein GLI2
Protein Accession Number:
NP_005261.2
Protein Gi Number:
86991432
Ncbi Gene Id Number:
2736
Background Reference 1:
Haycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK. Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function. PLoS Genet. 2005 Oct;1(4):e53. Epub 2005 Oct 28.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-674

List Size:
0.1 mg

List Price:

$430.00

Out of stock

Datasheet
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