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Western blot analysis of GLIPR1 in human small intestine tissue lysate with GLIPR1 antibody at 1 μg/ml.
Immunoflorescence of GLIPR1 in human small intestine tissue with GLIPR1 antibody at 5 μg/mL.
Western blot analysis of GLIPR1 in human small intestine tissue lysate with GLIPR1 antibody at 1 μg/ml.
Trial Size

GLIPR1 Antibody

CATALOG NUMBER: 7623

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
GLIPR1 antibody was raised against an 18 amino acid peptide near the center of human GLIPR1.
The immunogen is located within amino acids 60 - 110 of GLIPR1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
GLIPR1 antibody can be used for detection of GLIPR1 by Western blot at 1 - 2 μg/ml. For Immunoflorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GLIPR1 antibody is human and mouse reactive. At least two isoforms of GLIPR1 are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other GLIPR or GLIPR-like proteins.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 29 kDa
Observed: 29 kDa
Purification:
GLIPR1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GLIPR1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GLIPR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
GLIPR1
Additional Names:
GLIPR1 Antibody: GLIPR, RTVP1, CRISP7, GLIPR, Glioma pathogenesis-related protein 1, Protein RTVP-1, GliPR 1
Protein Accession Number:
NP_006842
Protein Gi Number:
110825980
Ncbi Gene Id Number:
11010
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The glioma pathogensis-related protein 1 (GLIPR1) is similar to both the pathogenesis-related protein (PR) superfamily and the cysteine-rich secretory protein (CRISP) family (1). GLIPR1 is a tumor suppressor whose expression is regulated by p53; its increased expression is associated with myelomocytic differentiation in macrophages, whereas decreased expression of this gene through gene methylation is associated with prostate cancer (2). The GLIPR1 gene is part of a p53 target gene cluster that includes GLIPR1L1 and GLIPR1L2, two highly homologous proteins whose expression patterns vary (3).
Background Reference 1:
Murphy EV, Zhang Y, Zhu W, et al. The human glioma pathogenesis-related protein is structurally related to pathogenesis-related proteins and its gene is expressed specifically in brain tumors. Gene 1995; 159:131-5.
Background Reference 2:
Ren C, Li L, Yang G, et al. RTVP-1, a tumor suppressor inactivated by methylation in prostate cancer. Cancer Res. 2004; 64:969-76.
Background Reference 3:
Ren C, Ren CH, Li L, et al. Identification and characterization of RTVP1/GLIPR1-like genes, a novel p53 target gene cluster. Genomics 2006; 88:163-72.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7623

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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