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Western blot analysis of GLIPR1L1 in human testis tissue lysate with GLIPR1L1 antibody at 1 μg/ml.
Immunohistochemistry of GLIPR1L1 in human testis tissue with GLIPR1L1 antibody at 5 μg/mL.
Immunofluorescence of GLIPR1L1 in human testis tissue with GLIPR1L1 antibody at 20 μg/mL.
Western blot analysis of GLIPR1L1 in human testis tissue lysate with GLIPR1L1 antibody at 1 μg/ml.
Trial Size

GLIPR1L1 Antibody

CATALOG NUMBER: 7625

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
GLIPR1L1 antibody was raised against an 18 amino acid peptide near the amino terminus of human GLIPR1L1 .
The immunogen is located within amino acids 20 - 70 of GLIPR1L1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GLIPR1L1 antibody can be used for detection of GLIPR1L1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GLIPR1L1 antibody is human specific. At least two isoforms of GLIPR1L1 are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other GLIPR or GLIPR-like proteins.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
Predicted: 26 kDa
Observed: 23 kDa
Purification:
GLIPR1L1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GLIPR1L1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GLIPR1L1 antibody can be stored at 4°C, stable for up to one year.
Ncbi Official Symbol:
GLIPR1L1
Additional Names:
GLIPR1L1 Antibody: PRO7434, ALKN2972, GLIPR1-like protein 1
Protein Accession Number:
NP_689992
Protein Gi Number:
22749527
Ncbi Gene Id Number:
256710
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The GLIPR1-like 1 protein (GLIPR1L1) gene is part of a p53 target gene cluster that includes the related proteins GLIPR1 and GLIPR1L2 (1). GLIPR1L1 is similar to both the pathogenesis-related protein (PR) superfamily and the cysteine-rich secretory protein (CRISP) family (2). GLIPR1 is a tumor suppressor whose expression is regulated by p53 (3). Unlike GLIPR1, GLIPR1L1 is expressed primarily in the testis and is thought to be involved in the binding of sperm to the oocyte complex (4).
Background Reference 1:
Ren C, Ren CH, Li L, et al. Identification and characterization of RTVP1/GLIPR1-like genes, a novel p53 target gene cluster. Genomics 2006; 88:163-72.
Background Reference 2:
Murphy EV, Zhang Y, Zhu W, et al. The human glioma pathogenesis-related protein is structurally related to pathogenesis-related proteins and its gene is expressed specifically in brain tumors. Gene 1995; 159:131-5.
Background Reference 3:
Ren C, Li L, Yang G, et al. RTVP-1, a tumor suppressor inactivated by methylation in prostate cancer. Cancer Res. 2004; 64:969-76.
Background Reference 4:
Gibbs GM, Lo JC, Nixon B, et al. Glioma pathogenesis-related 1-like 1 is testis enriched, dynamically modified, and redistributed during male germ cell maturation and has a potential role in sperm-oocyte binding. Endocrinology 2010; 151:2332-42.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7625

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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