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43-439 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-439 (1ug/ml) staining of Rat (A) and (0.1ug/ml) Pig (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-439 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue
43-439 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
43-439 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GLP1R Antibody

CATALOG NUMBER: 43-439

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QHQWDGLLSYQD
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Hippocampus and in Rat and Pig Brain lysates (calculated MW of 53.0kDa according to Human NP_002053.3, 52.9kDa according to Rat NP_036860.1 and 52.7kDa according to Pig NP_001243523.1). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificity:
The immunizing peptide represents part of an extracellular region.
Predicted Molecular Weight:
Approx 55kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GLP1R
Additional Names:
Glp1r, glucagon-like peptide 1 receptor, GLP-1R, GLP1Rc, GLP-1 receptor, GLP-1-R
Protein Accession Number:
NP_002053.3
Protein Gi Number:
166795283
Ncbi Gene Id Number:
2740
Background Reference 1:
Lamont BJ, Li Y, Kwan E, Brown TJ, Gaisano H, Drucker DJ.Pancreatic GLP-1 receptor activation is sufficient for incretin control of glucose metabolism in mice.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-439

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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