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42-524 (0.03ug/ml) staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-524 (3.75ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-524 (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-524 (0.03ug/ml) staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GLUD1 / GLUD2 Antibody

CATALOG NUMBER: 42-524

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ESEEQKRNRVRGILR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration: 3-5ug/ml.Immunofluorescence: Customer found particulate cytoplasm staining in HeLa.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Specificity:
This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GLUD1 / GLUD2
Additional Names:
GLUD1, glutamate dehydrogenase 1, GDH, GDH1, GLUD, MGC132003,glutamate dehydrogenase (NAD(P)+), GLUD2, glutamate dehydrogenase 2, GDH2, GLUDP1, glutamate dehydrogenase pseudogene 1
Protein Accession Number:
NP_005262.1,NP_036216.2
Protein Gi Number:
4885281
Ncbi Gene Id Number:
2746;2747

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-524

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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