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Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser831.
Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser831.

GluR1 (phospho Ser831) Antibody

CATALOG NUMBER: XPS-1012

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GluR1 (Ser831) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser831 of rat GluR1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Immunolabeling of the GluR1 protein in Western blots of rat brain is blocked by the Ser831 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
GluR1 antibody is specific for GluR1 phosphorylated at Ser831.
Predicted Molecular Weight:
100
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
Gria1
Protein Accession Number:
P19490
Protein Gi Number:
97536283
Ncbi Gene Id Number:
50592
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct subunits GluR1-4 and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser831. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.
Background Reference 1:
Lee, H.K., Takamiya, K., Han, J.S., Man, H.Y., Kim, C.H., Rumbaugh, G., Yu, S., Ding, L., He, C., Petralia, R.S., Wenthold, R.J., Gallagher, M. and Huganir, R.L., "Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory," Cell 112 (2003) 631 - 643.
Background Reference 2:
Banke, T.G., Bowie, D., Lee, H.K., Huganir, R.L., Schousboe, A., and Traynelis, S.F. "Control of GluR1 AMPA receptor function by cAMP-dependent protein kinase," J. Neurosci. 20 (2000) 89 - 102.
Background Reference 3:
Barria, A., Muller, D., Derkach, V., Griffith, L.C. and Soderling, T.R., "Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation," Science 276 (1997) 2042 - 2045.
Background Reference 4:
Roche, K.W., O'Brien, R.J., Mammen, A.L., Bernhardt, J., and Huganir, R.L., "Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit," Neuron, 16 (1996) 1179 - 1188.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1012

List Size:
0.15 mL

List Price:

$540.00

Datasheet
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