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Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845.
Western blot of rat hippocampal lysate showing phospho-specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845.

GluR1 (phospho Ser845) Antibody

CATALOG NUMBER: XPS-1013

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GluR1 (Ser845) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser845 of rat GluR1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Immunolabeling of the GluR1 protein in western blots of rat brain is blocked by the Ser845 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blots (WB). Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for IF, IHC and WB.
Specificity:
GluR1 antibody is specific for GluR1 phosphorylated at Ser845.
Predicted Molecular Weight:
100
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
Gria1
Protein Accession Number:
P19490
Protein Gi Number:
97536283
Ncbi Gene Id Number:
50592
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of 4 distinct subunits (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser845. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.
Background Reference 1:
Roche, K.W., O'Brien, R.J., Mammen, A.L., Bernhardt, J., and Huganir, R.L., "Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit," Neuron, 16 (1996) 1179 - 1188.
Background Reference 2:
Blackstone, C.D., Moss, S.J., Martin, L.J., Levey, A.I., Price, D.L. and Huganir, R.L., "Biochemical characterization and localization of a non-N- methyl-D-aspartate glutamate receptor in rat brain," J. Neurochem. 58 (1992) 1118 - 1126.
Background Reference 3:
McGlade-Mcculloh, E., Yamamoto, H., Tan, S.-E., Brickey, D.A. and Soderling, T.R. "Phosphorylation and regulation of glutamate receptors by calcium/calmodulin-dependent protein kinase II," Nature (London) 362 (1993) 640 - 642.
Background Reference 4:
Barria, A., Muller, D., Derkach, V., Griffith, L.C. and Soderling, T.R., "Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation," Science 276 (1997) 2042 - 2045.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1013

List Size:
0.15 mL

List Price:

$540.00

Datasheet
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