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Western blot analysis of extracts of various cell lines, using GLUT4 antibody (23-070) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse heart using GLUT4 antibody (23-070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using GLUT4 antibody (23-070) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using GLUT4 antibody (23-070) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using GLUT4 antibody (23-070) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

GLUT4 Antibody

CATALOG NUMBER: 23-070

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human GLUT4 (NP_001033.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
NIH/3T3
Positive Control 3:
HepG2
Positive Control 4:
Mouse kidney
Positive Control 5:
Mouse heart
Positive Control 6:
Rat heart
Predicted Molecular Weight:
Observed: 54kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC2A4
Additional Names:
SLC2A4, solute carrier family 2 (facilitated glucose transporter), member 4, insulin-responsive glucose transporter type 4, GLUT4, glucose transporter 4
Ncbi Gene Id Number:
6517
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-070

List Size:
100 uL

List Price:

$436.00

Datasheet
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