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Glycine Receptor Antibody
Glycine Receptor Antibody

Glycine Receptor Antibody

CATALOG NUMBER: XPS-2015

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
Glycine Receptor polyclonal antibody was raised against a peptide derived from the amino terminus of the *1 subunit of Glycine Receptor.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Glycine Receptor antibody is specific immunolabeling of the ~48k *1 and *2 subunits of the receptor in Western blots of rat spinal cord and in cell extracts containing the recombinant human glycine receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the *1 subunit of the receptor. Applications include Dot Blots (DB) and Western Blots (WB). Rabbit anti-Glycine Receptor recognizes glycine receptor α1 and α2 in rat spinal cord and brain stem. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Predicted Molecular Weight:
48
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Glycine Receptor antibody can be stored at -20°C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20°C. This product is stable at -20°C for at least 1 year.
Ncbi Official Symbol:
Glra1
Protein Accession Number:
P07727
Protein Gi Number:
12230887
Ncbi Gene Id Number:
25674
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gamma-amGlycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission. The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin. Gene targeting in mice showed that gephyrin is required for synaptic clustering of glycine receptors in spinal cord. The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins. These receptors have also been extremely useful in studies of synaptic clustering of receptors. During postnatal motoneuron development, the glycine receptor alpha subunit changes from alpha2 (fetal) to alpha1 (adult).
Background Reference 1:
Schofield, C.M., Jenkins, A. and Harrison, N.L., "A highly conserved aspartic acid residue in the signature disulfide loop of the a1 subunit is a determinant of gating in the glycine receptor," J. Biol. Chem. 278 (2003) 34,079-34,083.
Background Reference 2:
Meier, J., Vannier, C., Serge, A., Triller, A., and Choquet, D. "Fast and reversible trapping of surface glycine receptors by gephyrin," Nat. Neurosci. 4 (2001) 253 - 260.
Background Reference 3:
Beckstead, M.J., Weiner, J.L, Eger, E.I., Gong, D.H. and Mihic, S.J., "Glycine and gamma-aminobutyric acid(A) receptor function is enhanced by inhaled drugs of abuse," Mol. Pharmacol. 57 (2000) 1199 - 1205.
Background Reference 4:
Eggers, E.D., O'Brien, J.A., and Berger, A.J., "Developmental changes in the modulation of synaptic glycine receptors by ethanol," J. Neurophysiol. 84 (2000) 2409 - 2416.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-2015

List Size:
200 ug

List Price:

$540.00

Datasheet
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