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IHC testing of FFPE human fetal liver tissue with Glypican-3 antibody. HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.
IHC testing of FFPE human fetal liver tissue with Glypican-3 antibody. HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.

Glypican 3 Antibody

CATALOG NUMBER: 33-640

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
A partial human protein was used as the immunogen for the Glypican-3 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the Glypican-3 antibody should be determined by the researcher.
Purification:
Protein A affinity chromatography
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
GPC3
Additional Names:
Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5
Ncbi Gene Id Number:
2719
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Glypican-3 is a proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-640

List Size:
100 ug

List Price:

$626.00

Datasheet
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