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Human Glypican 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human FGF basic, Tag Free at 0.5 ug/mL (100 uL/well) can bind Human Glypican 3, His Tag with a linear range of 8-31 ng/mL (QC tested).
Immobilized Human Glypican 3, His Tag at 0.5 ug/mL (100 uL/well) can bind Anti-Human GPC3 MAb with a linear range of 0.1-2 ng/mL (Routinely tested).
Human Glypican 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Glypican 3 / GPC3 Recombinant Protein

CATALOG NUMBER: 11-400

Tested Applications:
ELISA, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Gln 25 - His 559
Fusion Tag:
His Tag
Tested Applications:
ELISA, WB
Application Note:
This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 355-RQYR-358, and will be partially processed into N and C-terminal fragment with calculated MW of 38.1 kDa and 24.6 kDa respectively
Predicted Molecular Weight:
38.1 kDa and 24.6 kDa
Purity:
>90% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
GPC3
Additional Names:
GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
Protein Accession Number:
NP_004475.1
Ncbi Gene Id Number:
2719
Background:
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
Background Reference 1:
Davoodi J., et al., 2007, Proteomics 7:2300-2310.
Background Reference 2:
Veugelers M., et al., 2000, Hum. Mol. Genet. 9:1321-1328.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

11-400

List Size:
0.1 mg

List Price:

$540.00

Datasheet
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