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To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Murine GM-CSF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.
To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Murine GM-CSF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.

GM-CSF Antibody

CATALOG NUMBER: 38-330

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mGM-CSF. Anti-Murine GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine GM-CSF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF.
Western Blot
To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
GM-CSF specific antibody was purified by affinity chromatography employing immobilized mGM-CSF matrix
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Csf2
Additional Names:
Granulocyte-Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-alpha
Protein Accession Number:
P01587
Protein Gi Number:
P01587.1
Ncbi Gene Id Number:
12981
User Note:
Centrifuge vial prior to opening.
Background:
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Murine GM-CSF is a 14.2 kDa globular protein consisting of 125 amino acid residues.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-330

List Size:
0.05 mg

List Price:

$391.00

Datasheet
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