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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human GM2A with DYKDDDDK tag probed with 42-413 (1.0ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/30000) in lane C. Mock-transfected HEK293 probed with 42-413 (1mg/ml) in Lane B. Primar
42-413 (0.3ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human GM2A with DYKDDDDK tag probed with 42-413 (1.0ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/30000) in lane C. Mock-transfected HEK293 probed with 42-413 (1mg/ml) in Lane B. Primar

GM2A Antibody

CATALOG NUMBER: 42-413

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: KRLGCIKIAAS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:In transfected HEK293 transiently expressing full-length Human GM2A (myc and DYKDDDDK tagged), a band of approx. 28kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Approx. 24kDa band was observed in Rat Kidney lysates (calculated MW of 21.5kDa according to NP_758838.2 ). Recommended concentration: 0.3-1ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.
Specificity:
This antibody is expected to recognize isoform 1 (NP_000396.2) only.
Positive Control 1:
Transfected HEK293
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GM2A
Additional Names:
GM2A, GM2 ganglioside activator, SAP-3, cerebroside sulfate activator protein, sphingolipid activator protein 3
Protein Accession Number:
NP_000396.2
Protein Gi Number:
39995109
Ncbi Gene Id Number:
2760

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-413

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

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