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Western blot analysis of in HepG2 cell line lysates (35ug/lane)
GMNN Antibody (Center Y111) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of in HepG2 cell line lysates (35ug/lane)

GMNN Antibody

CATALOG NUMBER: 61-843

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This GMNN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-123 amino acids from the Central region of human GMNN.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GMNN
Additional Names:
Geminin, GMNN
Protein Accession Number:
O75496
Ncbi Gene Id Number:
51053
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GMNN inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
Background Reference 1:
De Marco, V., et al. Proc. Natl. Acad. Sci. U.S.A. 106(47):19807-19812(2009)
Background Reference 2:
Zhu, W., et al. Cancer Res. 69(11):4870-4877(2009)
Background Reference 3:
Lu, F., et al. Biol. Cell 101(5):273-285(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-843

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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