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43-697 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-697 (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-697 (1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

GNA12 Antibody

CATALOG NUMBER: 43-697

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KKHFPDFRGDPHR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in Human Placenta lysates and approx 42kDa in Mouse Heart lysates (calculated MW of 37.6kDa according to Human NP_001269370.1 and 44.1kDa according to Mouse NP_034432.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_031379.2; NP_001269369.1; NP_001269370.1; NP_001280021.1).
Predicted Molecular Weight:
Approx 37kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GNA12
Additional Names:
GNA12, guanine nucleotide binding protein (G protein) alpha 12, NNX3, RMP, gep, G-protein subunit alpha-12, WUGSC:H_GS165O14.2, g alpha-12, guanine nucleotide-binding protein subunit alpha-12
Protein Accession Number:
NP_031379.2,NP_001269369.1,NP_001269370.1,NP_001280021.1
Protein Gi Number:
42476111
Ncbi Gene Id Number:
2768
Background Reference 1:
Cox B, Sharma P, Evangelou AI, Whiteley K, Ignatchenko V, Ignatchenko A, Baczyk D, Czikk M, Kingdom J, Rossant J, Gramolini AO, Adamson SL, Kislinger T.Translational analysis of mouse and human placental protein and mRNA reveals distinct molecular pathologies in human preeclampsia.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-697

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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