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43-712 (2ug/ml) staining of Human Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-712 (5ug/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-712 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-712 (2ug/ml) staining of Human Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

GNAI2 Antibody

CATALOG NUMBER: 43-712

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PEYTGANKYDE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 40.5kDa according to NP_002061.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thyroid and Tonsil. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_002061.1; NP_001159897.1).
Predicted Molecular Weight:
Approx 38kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GNAI2
Additional Names:
GNAI2, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1, GTP-binding regulatory protein Gi alpha-2 chain, adenylate cyclase-inhibiting G alpha protein, guanine nucleotide-binding protein G(i) subunit alpha-2, guanine nucleotide-binding protein G(i), alpha-2 subunit
Protein Accession Number:
NP_002061.1,NP_001159897.1
Protein Gi Number:
4504041
Ncbi Gene Id Number:
2771
Background Reference 1:
Kremer KN, Kumar A, Hedin KE.G alpha i2 and ZAP-70 mediate RasGRP1 membrane localization and activation of SDF-1-induced T cell functions.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-712

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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